preparation of an artificial matrix to replace human standards of prostate specific antigen irma assay kit [persian]

نویسندگان

haleh foroutan

reza najafi

mohammad hossein babaei

چکیده

introduction: prostate specific antigen (psa) is one of the most sensitive markers for diagnosis of prostate cancer. immunoradiometric kit (irma) is a common and sensitive method for determination of psa in clinical laboratories. this kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with i-125, series of standards in different ranges of concentration and lyophilized control samples. in irma method, psa is determined in human serum. therefore, matrix of standards have to be similar to human serum. using human serum as standard has many shortcomings namely biohazard contamination, instability, serum protein precipitation and unavailability. artificial matrix however, is an ideal substitute for human serum. methods: in this study, we used an artificial matrix as standard sample and evaluated its effects including reproducibility, sensitivity, precision and stability for determination of psa in comparision to human serum. results: the specificity, sensitivity, storage condition and the stability of these standards prepared with artificial matrix were studied. similar results in comparison to human serum was observed. conclusion: the prepared standards with this matrix had suitable and ideal properties and it can be used as standard in psa assay kit.

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Preparation of an artificial matrix to replace human standards of prostate specific antigen IRMA assay kit [Persian]

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preparation of prostate specific antigen standards for immunoradiometric assay

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عنوان ژورنال:
iranian journal of nuclear medicine

ناشر: tehran university of medical sciences

ISSN 1681-2824

دوره 14

شماره 1 2006

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